PhysioMar 2012

 

 

PHYSIOMAR 12

CALL FOR ABSTRACTS

Physiomar 12 will be held in Santiago de Compostela, Galicia (Spain) from 4th to 8th September, 2012 at the Faculty of Biology (Room Santiago Ramón y Cajal), University of Santiago de Compostela. The conference is being organised by USC (Universidade de Santiago de Compostela), Instituto de Investigaciones Marinas (IIM-CSIC) and Centro de Investigacións Mariñas (CIMA, Xunta de Galicia).

Physiomar 12 is planned as a forum for the communication of recent advances in all aspects of the physiology of marine molluscs and will focus mainly on Reproduction, Growth and Bioenergetics, Nutrition, Biomolecules and Biomaterials, and Genetics, genomics and proteomics. The 2012 conference will also include two specific sessions on Inmune reponse and Response to environmental changes and toxicity.

 Call for Abstracts - the deadline to submit an abstract is April 15, 2012.

All abstracts, both oral and poster, will be published in the book of abstracts.  All student presentations, both oral and poster, will be entered into the Student Presentation Awards. Student must be the presenter author.

You are invited to submit an abstract for an oral or poster presentation on any Physiomar 12 related topic, including:

Reproduction
Growth and Bioenergetics
Nutrition
Biomolecules and Biomaterials
Genetics, Genomics and Proteomics
Inmune Reponse
Response to Environmental Changes and Toxicity

Abstracts should be submitted in English no later than 15th April 2012. Abstracts MUST be submitted by email to physiomar12@gmail.com as .rtf files, following the detailed format specifications provided in Instructions to authors (see below). Please indicate Physiomar 12 and a topic in the subject. The abstract should not exceed one full page. All oral presentations must be PowerPoint and must given to the technical staff by 5 pm the day before the presentation. A permanent poster exhibition will be displayed at the workshop centre. The size of the poster should not exceed 110 cm high/ 80 cm wide so as to be compatible with the panels of the poster stands. Please state your preference for an oral or poster presentation. The scientific committee reserves the right to ask that an oral presentation be changed to a poster or vice versa, to help ensure the organisation of balanced thematic sessions. After submitting, you will receive an email confirming the reception of your abstract.

Registration will open 3th May 2012 and information about will soon be posted on the Physiomar 12 web site. For information on planning your trip and about the meeting the Physiomar 12 web site should be functional by the beginning of February, 2012. 

We are looking forward to seeing you in Santiago de Compostela, Galicia, Spain.

The Organising Committee:

Luz Pérez-Parallé (Universidade de Santiago de Compostela)
José Luís Sánchez (Universidade de Santiago de Compostela)
Antonio Pazos (Universidade de Santiago de Compostela)
Jose Babarro (Instituto de Investigaciones Marinas, IIM-CSIC)
Antonio Villalba (Centro de Investigacións Mariñas, Xunta de Galicia)
José Miguel Fuentes (Centro de Investigacións Mariñas, Xunta de Galicia)

 

See instructions to authors below

 

Title of communication

Name of author (First name and LAST NAME)1 and co-author(s) (First name and LAST NAME)2

     1. Address of organisation

     2. Address of organisation

     Email (speaker only):

Keywords: molluscs, growth...etc

 

Instructions

The abstract should not exceed one full page (including any graphs and figures) and must be submitted in English in electronic format as an .rtf file (Microsoft Word, Open Office) to: physiomar12@gmail.com

Each abstract should include:

  • A precise, self explanatory title
  • Name(s) of first author and co-author(s) (the name of the speaker underlined)
  • Affiliations of all authors, with addresses, and the e-mail address of the speaker.
  • Keywords (5 maximum).
  • One table or one figure.

Formatting

Formatting should be as follows:

  • Paper: A4 format
  • Margins: upper 2.5 cm, lower 2.5 cm, left 2.5 cm, right 2.5 cm
  • Line spacing: single
  • Font: Times New Roman
  • Character size:

               title, 14, bold, centered
               authors, 11, regular, (last name CAPS), centered
               organization, 11, regular, left
               Email, 11, regular, left
               keywords, 11, italic, centred
               text, 11, regular, justified.
               paragraphs: separated by single-line space

 

 

Example Abstract submission: 

Hox genes in the black scallop Chlamys varia (L.)

Crimgilt MESÍAS-GANSBILLAR, Antonio J. PAZOS, José L. SÁNCHEZ and M. Luz PÉREZ-PARALLÉ

Laboratorio de Biología Molecular y del Desarrollo. Departamento de Bioquímica y Biología Molecular. Instituto de Acuicultura. Universidad de Santiago de Compostela. Spain

Email: luz.perez-paralle@usc.es

 

Keywords: homeobox genes, caudal, Gbx, Lox5, Posterior Hox genes

 

Homeobox genes (Hox genes) encode a family of transcription factors that have essential roles in the regulation of development and differentiation in eukaryotes. Although they have been extensively studied in different phyla, relatively little is known about homeobox-containing genes and their function in molluscs. These transcription factors present the homeodomain, a highly conserved DNA-binding motif of 60 amino acids and encoded by a short 180 bp DNA fragment, the homeobox. The conservation of this motif and its proliferation in metazoans emphasizes the importance of the role of homeobox genes in development and differentiation.

In previous studies we analyzed the homeobox-containing genes of the bivalve mollusc Pecten maximus (L.) and we demonstrated the presence of proboscipedia and caudal gene homologues. We also analyzed the homeobox-containing genes of the bivalve mollusc Mytilus galloprovincialis (Lmk.) and have demonstrated the presence of Hox genes belonging to the anterior, PG3, and central classes. Recently we have identified two cdx and a Gbx gene fragments in the oyster, Ostrea edulis (L.) and a lab gene from the clamp, Venerupis pullastra (Montagu).

We have designed a pair of degenerate oligonucleotide primers that correspond to conserved homeodomain sequences. Genomic DNA was isolated from adult black scallops. DNA was amplified using 0.5mM of each primer and Hot Master Taq DNA Polymerase. PCR was performed at 95ºC (5 min) and then at 95ºC (1 min), 40ºC (1 min), 72ºC (30 s) for 39 cycles, and finally 10 min at 72ºC. Positive PCR products were reamplified and later cloned in the pGem-T Easy Vector System II (Promega). DNA of individual clones, obtained using GenElute Plasmid Miniprep Kit (Sigma), was double strand sequenced by using ABI Prism dRhodamine Terminator Cycle Sequencing kit.

In this study we have identified the first homeobox genes in a bivalve mollusc, the black scallop Chlamys varia (L.). Following PCR amplification of Chlamys varia DNA, we were able to recover two Gbx gene fragments from the non-Hox/ParaHox class; the derived amino acid sequence was designated Cvox Gbx (23 aa) and Cvox Gbx1 (72 aa) from Chlamys varia homeobox. We also isolated and analyzed two ParaHox genes, caudal gene homologues. The derived amino acid sequences (23 aa) were designated Cvox Cad and Cvox Cad1. In this study we also report the isolation of a Lox5 gene fragment (Cvox Lox5), ftz gene orthologous. Finally, we have identified two posterior Hox genes (Cvox Post-1 and Cvox Post-2).

Comparison of the Cvox homeodomain with those of several homeodomain proteins reveals a high level of conservation. Molecular alignments and phylogenetic analysis indicate that these scallop genes are homologues of the caudal ParaHox, GBX-class, Lox5 and Posterior Hox genes.